Rank | Heat | Nation | Athletes | Time | Notes |
1 | 1 | United States | Nathan Strother, Josephus Lyles, Paul Dedewo, Je'von Hutchison | 3:02.06 | Q |
2 | 3 | Trinidad and Tobago | Deon Lendore, Jereem Richards, Asa Guevara, Machel Cedenio | 3:02.49 | Q, SB |
3 | 2 | Japan | Julian Jrummi Walsh, Yoshinobu Imoto, Kentaro Sato, Kota Wakabayashi | 3:02.55 | Q, SB |
4 | 1 | Jamaica | Demish Gaye, Akeem Bloomfield, Javon Francis, Nathon Allen | 3:02.67 | Q, SB |
5 | 3 | South Africa | Gardeo Isaacs, Ranti Dikgale, Pieter Conradie, Ashley Hlungwani | 3:02.77 | Q |
6 | 1 | Great Britain | Rabah Yousif, Dwayne Cowan, Cameron Chalmers, Martyn Rooney | 3:02.67 | q, SB |
7 | 1 | Australia | Alex Beck, Tyler Gunn, Murray Goodwin, Steven Solomon | 3:03.53 | q, SB |
8 | 2 | Belgium | Dylan Borlée, Robin Vanderbemden, Julien Watrin, Jonathan Sacoor | 3:03.70 | Q, SB |
9 | 1 | Czech Republic | Jan Tesař, Pavel Maslák, Vít Müller, Michal Desenský | 3:03.72 | qB, SB |
10 | 1 | Italy | Daniele Corsa, Michele Tricca, Edoardo Scotti, Alessandro Sibilio | 3:03.97 | qB, SB |
11 | 2 | France | Mame-Ibra Anne, Thomas Jordier, Ludvy Vaillant, Fabrisio Saïdy | 3:04.11 | qB, SB |
12 | 3 | Netherlands | Ramsey Angela, Terrence Agard, Liemarvin Bonevacia, Tony van Diepen | 3:04.30 | qB, SB |
13 | 3 | China | Feng Zhiqiang, Wu Lei, Yang Lei, Wu Yuang | 3:04.85 | qB |
14 | 3 | Poland | Dariusz Kowaluk, Karol Zalewski, Przemysław Waściński, Tymoteusz Zimny | 3:04.90 | qB, SB |
15 | 2 | Colombia | Bernardo Baloyes, Yilmar Herrera, Jhon Alexander Solís, Diego Palomeque | 3:05.55 | qB, SB |
16 | 2 | Germany | Marc Koch, Patrick Schneider, Manuel Sanders, Johannes Trefz | 3:06.03 | qB, SB |
17 | 2 | India | Kunhu Muhammed Puthanpura, Jithu Baby, Jeevan Karekoppa Suresh, Muhammed Anas | 3:06.05 | |
| 3 | Bahamas | | DNS | |